EliKine™ Human β-hCG ELISA Kit employs a two-site sandwich ELISA to quantitate β-hCG in samples. An antibody specific for β-hCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any β-hCG present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for β-hCG is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of β-hCG bound in the initial step. The color development is stopped and the intensity of the color is measured.
The EliKine™ β-hCG Human ELISA Kit contains below components:
Human β-hCG microplate
Human β-hCG standard
Human β-hCG detect antibody
Urine Sample diluent
HRP substrate A
HRP substrate B
Stop solution
Wash buffer
Plate covers.
The Human β-hCG ELISA Kit with the calibration range is: 8 IU/L-240 IU/L, and sensitivity is: 2.0 IU/L.
More Abbkine EliKine™ series of ELISA Kits at EliKine™.
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