Estradiol is an estrogenic hormone, C18H24O2, produced by the ovaries and used in treating estrogen deficiency. Estradiol is essential for maintaining normal female functions and is normally produced by the ovaries. After menopause, the ovaries decrease their production of this hormone.Estradiol is a sex hormone. Estradiol is the predominant sex hormone present in females; however, it is present in males, albeit at lower levels, as well. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including the bones.Estradiol is produced not only in the gonads: In both sexes, precursor hormones, to be specific testosterone, are converted by aromatization to estradiol. In particular, fat cells are active to convert precursors to estradiol, and will continue to do so even after menopause.
Rat Estradiol antibody (E2-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated E2-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled E2-Ab and unlabeled E2-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Rat Estradiol antibody (E2-Ab) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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