Heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of proteins. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function.Diphtheria toxin inhibits protein synthesis in eukaryotic cells by catalyzing inactivation of elongation factor-2 (EF2). Entry of toxin into cells is dependent on binding to the cell surface.Mice and rats are resistant to diphtheria toxin, and the resistance extends to cultured cells. Mouse-human hybrid cells are sensitive to diphtheria toxin. Creagan et al. (1975) showed that sensitivity is determined by human chromosome 5, which presumably carries a gene for a receptor for toxin.
Rat Heparin-binding EGF-like growth factor (HBEGF) ELISA Kit employs a two-site sandwich ELISA to quantitate HBEGF in samples. An antibody specific for HBEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHBEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HBEGF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HBEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Heparin-binding EGF-like growth factor (HBEGF) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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