Rat Serine/threonine-protein kinase DCLK1 (DCLK1) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

Dclk1 encodes a member of the protein kinase superfamily and the doublecortin family. The typical protein encoded by Dclk1 contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the protein is independent of its protein kinase activity. Dclk1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the full-length nature of the variant produced from the 5' promoter has not been determined. Current reference sequence data represents two alternatively spliced transcript variants produced from the 3' promoter and their protein products lack the doublecortin domain.

Rat Serine/threonine-protein kinase DCLK1 (DCLK1) ELISA Kit employs a two-site sandwich ELISA to quantitate DCLK1 in samples. An antibody specific for DCLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCLK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Serine/threonine-protein kinase DCLK1 (DCLK1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/rat-serine-threonine-protein-kinase-dclk1-dclk1-elisa-kit-kte100790/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.