Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. MEP1B encodes the beta subunit. Targeted disruption of MEP1B in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine.
Rat Meprin A subunit beta (MEP1B) ELISA Kit employs a two-site sandwich ELISA to quantitate MEP1B in samples. An antibody specific for MEP1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMEP1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEP1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEP1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Meprin A subunit beta (MEP1B) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/rat-meprin-a-subunit-beta-mep1b-elisa-kit-kte100635/
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