Ferritin heavy chain is a heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined.The functionality of the gene was demonstrated by the fact that both transient transfectants and stable transformants of mouse fibroblasts actively transcribed human ferritin heavy-chain mRNA.
Sheep Ferritin heavy chain (FTH1) ELISA Kit employs a two-site sandwich ELISA to quantitate FTH1 in samples. An antibody specific for FTH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFTH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FTH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FTH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sheep Ferritin heavy chain (FTH1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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