Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit,Abbkine_specification/price/image

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POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.A second site of hybridization to 11q23.1 was thought to represent a pseudogene, most likely a retroposon, since no remnants of introns could be identified in the study of genomic DNA.

Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR2E in samples. An antibody specific for POLR2E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLR2E present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR2E is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR2E bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.