ADM2 belongs to a family of calcitonin related peptide hormones important for regulating diverse physiologic functions and the chemical composition of fluids and tissues. ADM2 contains a dibasic cleavage site, followed by a 47-amino acid mature peptide hormone sequence and an alpha-amidation donor glycine at the C terminus.Immunohistochemical analysis of several mouse tissues detected Adm2 in pituitary and stomach. PCR analysis of a human gastrointestinal panel detected ADM2 in almost all regions examined. Immunohistochemical staining showed ADM2 in the muscularis mucosae layer of stomach. Western blot analysis of transfected embryonic kidney cells found mature ADM2 secreted into the culture medium; the apparent molecular mass was about 5.0 kD.
Rat Adrenomedullin 2 (ADM2) ELISA Kit employs a two-site sandwich ELISA to quantitate ADM2 in samples. An antibody specific for ADM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Adrenomedullin 2 (ADM2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/rat-adrenomedullin-2-adm2-elisa-kit-kte100338/
bio-equip.cn
中文
