Vitamin C, also known as ascorbic acid and L-ascorbic acid, is a vitamin found in food and used as a dietary supplement. As a supplement it is used to treat and prevent scurvy.Evidence does not support use in the general population for the prevention of the common cold. It may be taken by mouth or by injection.It is generally well tolerated.Large doses may cause gastrointestinal discomfort, headache, trouble sleeping, and flushing of the skin. Normal doses are safe during pregnancy. Vitamin C is an essential nutrient involved in the repair of tissue.Foods that contain vitamin C include citrus fruit, tomatoes, red peppers, and potatoes.Vitamin C was discovered in 1912, isolated in 1928, and first made in 1933. It is on the World Health Organization's List of Essential Medicines, the most effective and safe medicines needed in a health system.Vitamin C is available as a generic medication and over the counter.In 2015, the wholesale cost in the developing world was about 0.003 to 0.007 USD per tablet. In some countries, ascorbic acid may be added to foods such as breakfast cereal.
Rat Vitamin C (VC) ELISA Kit employs a two-site sandwich ELISA to quantitate VC in samples. An antibody specific for VC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Vitamin C (VC) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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