Na+/K+-ATPase (sodium-potassium adenosine triphosphatase, also known as the Na+/K+ pump or sodium-potassium pump) is an enzyme (EC 3.6.3.9) (an electrogenic transmembrane ATPase) found in the plasma membrane of all animal cells. The Na+/K+-ATPase enzyme is a solute pump that pumps sodium out of cells while pumping potassium into cells, both against their concentration gradients. This pumping is active (i.e. it uses energy from ATP) and is important for cell physiology. An example application is nerve conduction.
Pig Na+/K+-ATPase (Na/K ATPase) ELISA Kit employs a two-site sandwich ELISA to quantitate Na/K ATPa in samples. An antibody specific for Na/K ATPa has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNa/K ATPa present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Na/K ATPa is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Na/K ATPa bound in the initial step. The color development is stopped and the intensity of the color is measured.
Pig Na+/K+-ATPase (Na/K ATPase) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/pig-na-k-atpase-na-k-atpase-elisa-kit-kte80201/
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