Mouse GDP-fucose protein O-fucosyltransferase 2 (POFUT2) ELISA Kit,Abbkine_specification/price/image

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POFUT2 is an enzyme responsible for adding fucose sugars in O linkage to serine or threonine residues in Thrombospondin repeats. The protein is an inverting glycosyltransferase, which means that the enzyme uses GDP-β-L-fucose as a donor substrate and transfers the fucose in O linkage to the protein producing fucose-α-O-serine/threonine.Almost all glycosyltransferases reside in the Golgi apparatus. However, POFUT2 as well as the related enzyme POFUT1 have recently been shown to reside in the endoplasmic reticulum.The deduced protein contains 428 amino acids. RT-PCR ELISA detected moderate expression in all adult and fetal tissues and specific brain regions examined. Crystal structures of POFUT2 reveal a variation of the classical GT-B fold and identify sugar donor and TSR acceptor binding sites.

Mouse GDP-fucose protein O-fucosyltransferase 2 (POFUT2) ELISA Kit employs a two-site sandwich ELISA to quantitate POFUT2 in samples. An antibody specific for POFUT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOFUT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POFUT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POFUT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse GDP-fucose protein O-fucosyltransferase 2 (POFUT2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.