Mouse Pogo transposable element with KRAB domain (POGK) ELISA Kit,Abbkine_specification/price/image

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The exact function of the protein encoded by POGK is not known. However, this gene product contains a KRAB domain (which is involved in protein-protein interactions) at the N-terminus, and a transposase domain at the C-terminus, suggesting that it may belong to the family of DNA-mediated transposons in human.The Krueppel-associated box (KRAB) is a domain of around 75 amino acids that is found in the N-terminal part of about one third of eukaryotic Krueppel-type C2H2 zinc finger proteins (ZFPs) . It is enriched in charged amino acids and can be divided into subregions A and B, which are predicted to fold into two amphipathic alpha-helices. The KRAB A and B boxes can be separated by variable spacer segments and many KRAB proteins contain only the A box.

Mouse Pogo transposable element with KRAB domain (POGK) ELISA Kit employs a two-site sandwich ELISA to quantitate POGK in samples. An antibody specific for POGK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOGK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POGK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POGK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Pogo transposable element with KRAB domain (POGK) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.