POLN is predicted to contain a fingers-thumb-palm structure conserved in the PolA family. Northern blot analysis of human tissues detected an approximately 4-kb transcript in testis, heart, and skeletal muscle, and a 2-kb transcript in heart, liver, skeletal muscle, kidney, and pancreas. The 2-kb transcript was detected in a panel of human cancer cell lines, and the 4-kb transcript was detected only in a chronic myelogenous leukemia cell line. Both POLN and DNA helicase HEL308 showed high expression in human and mouse testis. In situ hybridization of mouse testis tissue localized the highest Poln expression to meiotic spermatocytes and postmeiotic round spermatids, while Hel308 expression occurred in primary spermatocytes but was not detected in round spermatids.
Mouse DNA polymerase nu (POLN) ELISA Kit employs a two-site sandwich ELISA to quantitate POLN in samples. An antibody specific for POLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse DNA polymerase nu (POLN) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-dna-polymerase-nu-poln-elisa-kit-kte71448/
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