Ribonuclease P (RNase P) removes the 5-prime leader sequences from precursor tRNA molecules. RNase P consists of an RNA species (H1 RNA), the POP1 protein, and at least 7 proteins called RPPs. The RPPs have apparent molecular masses of 14 kD (RPP14), 20 kD (RPP20), 25 kD (RPP25), 29 kD (RPP29), 30 kD (RPP30), 38 kD (RPP38), and 40 kD (RPP40). Patients with scleroderma have serum reactive with RNase P, the Th antigen, which is also referred to as the To antigen, and RPP30 and RPP38.POP7 contains 140 amino acids with a predicted molecular mass of nearly 16 kD. Immunologic analysis determined that RPP20, unlike RPP30 and RPP38, is not a target for antisera from systemic sclerosis patients.
Mouse Ribonuclease P protein subunit p20 (POP7) ELISA Kit employs a two-site sandwich ELISA to quantitate POP7 in samples. An antibody specific for POP7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOP7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POP7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POP7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Ribonuclease P protein subunit p20 (POP7) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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