Mouse Sperm mitochondrial-associated cysteine-rich protein (SMCP) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

The MMP15 cDNA encodes a 669-amino acid protein that has the typical structural features of an MMP. In addition, it contains a predicted transmembrane segment at the C terminus. MMP15 shares 73.9% sequence similarity with MMP14, a membrane-localized MMP that also contains a C-terminal transmembrane segment. the membrane-type MMPs are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms. Will and Hinzmann showed by immunoblotting that MMP15-specific antibodies detect a 72-kD protein in lung cell surface membranes, suggesting that MMP15 is membrane-bound. They demonstrated by Northern blotting that MMP15 is widely expressed as a 3.6-kb transcript, with highest levels in liver, placenta, testis, colon, and intestine.

Mouse Sperm mitochondrial-associated cysteine-rich protein (SMCP) ELISA Kit employs a two-site sandwich ELISA to quantitate SMCP in samples. An antibody specific for SMCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMCP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMCP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMCP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Sperm mitochondrial-associated cysteine-rich protein (SMCP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/mouse-sperm-mitochondrial-associated-cysteine-rich-protein-smcp-elisa-kit-kte71421/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.