Mouse Keratinocyte autocrine factor/Amphiregulin (KAF/AR) ELISA Kit,Abbkine_specification/price/image

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The androgen receptor (AR) is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS).

Mouse Keratinocyte autocrine factor/Amphiregulin (KAF/AR) ELISA Kit employs a two-site sandwich ELISA to quantitate AREG in samples. An antibody specific for AREG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAREG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AREG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AREG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Keratinocyte autocrine factor/Amphiregulin (KAF/AR) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/mouse-keratinocyte-autocrine-factor-amphiregulin-kaf-ar-elisa-kit-kte71410/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.