Mouse Potassium-transporting ATPase subunit beta (ATP4B) ELISA Kit,Abbkine_specification/price/image

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ATP4b belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Hydrogen-potassium adenosine triphosphatase belongs to a family of P-type cation-transporting ATPases that also includes Ca(2+)-ATPase and Na(+),K(+)-ATPase. In gastric parietal cells, H(+),K(+)-ATPase plays an essential role in the formation of hydrochloric acid. Like the Na(+),K(+)-ATPase, H(+),K(+)-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit.

Mouse Potassium-transporting ATPase subunit beta (ATP4B) ELISA Kit employs a two-site sandwich ELISA to quantitate ATP4B in samples. An antibody specific for ATP4B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyATP4B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATP4B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATP4B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Potassium-transporting ATPase subunit beta (ATP4B) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.