Mouse Calpain-1 catalytic subunit (CAPN1) ELISA Kit,Abbkine_specification/price/image

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Calpain (calcium-dependent protease) is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I (mu-calpain) and calpain II (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 80 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit (CAPNS1), with the differences arising from the L subunits, L1 (CAPN1) and L2 (CAPN2). The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. Calpain-1 catalytic subunit is the large subunit of the ubiquitous enzyme, calpain 1.

Mouse Calpain-1 catalytic subunit (CAPN1) ELISA Kit employs a two-site sandwich ELISA to quantitate CAPN1 in samples. An antibody specific for CAPN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCAPN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAPN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAPN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Calpain-1 catalytic subunit (CAPN1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.