Doublecortin-like kinase 1 (Dclk1)  encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the biological validity of some variants has not been determined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.

Mouse Serine/threonine-protein kinase DCLK1 (DCLK1) ELISA Kit employs a two-site sandwich ELISA to quantitate DCLK1 in samples. An antibody specific for DCLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCLK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Serine/threonine-protein kinase DCLK1 (DCLK1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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