Doublecortin-like kinase 3 (Dclk3) encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions.
Mouse Serine/threonine-protein kinase DCLK3 (DCLK3) ELISA Kit employs a two-site sandwich ELISA to quantitate DCLK3 in samples. An antibody specific for DCLK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCLK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCLK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCLK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Serine/threonine-protein kinase DCLK3 (DCLK3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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