The protein encoded by MKRN3 contains a RING (C3HC4) zinc finger motif and several C3H zinc finger motifs. MKRN3 is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome. An antisense RNA of unknown function has been found overlapping MKRN3.
Mouse Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit employs a two-site sandwich ELISA to quantitate MKRN3 in samples. An antibody specific for MKRN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMKRN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MKRN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MKRN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-probable-e3-ubiquitin-protein-ligase-makorin-3-mkrn3-elisa-kit-kte71017/
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