Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) ELISA Kit,Abbkine_specification/price/image

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Complex II in mitochondria, of which succinate dehydrogenase (EC 1.3.99.1) is a component, has 4 subunits. In order of decreasing molecular mass, they are the flavoprotein (SDHA), the iron sulfur protein (SDHB), and the 2 integral membrane proteins, SDHC and SDHD.Kita et al. (1990) cloned and sequenced the iron sulfur protein subunit. A clone was isolated from a human liver cDNA library. The open reading frame encodes a 252-amino acid protein. The amino acid sequence showed approximately 94% homology with that of bovine heart. Au et al. (1995) described the complete genomic clone for the gene encoding the iron sulfur protein subunit.

Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) ELISA Kit employs a two-site sandwich ELISA to quantitate SDHB in samples. An antibody specific for SDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDHB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.