Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) ELISA Kit,Abbkine_specification/price/image

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TIM44, a peripheral inner membrane protein, tethers mitochondrial HSP70 to the import channel. A model peptide substrate rapidly released mitochondrial HSP70 from TIM44, even in the absence of ATP hydrolysis. In vivo, the analogous interaction of translocating polypeptide would release mitochondrial HSP70 from the channel.TIMM44 is hydrophilic and contains no hydrophobic membrane-spanning segments. The N terminus has a coiled-coil motif and a matrix-targeting segment with 6 positively and no negatively charged residues. Western blot analysis showed that TIMM44 is present in membranes as well as matrix and is most likely a 45-kD peripheral membrane protein loosely associated with the inner membrane of mitochondria.

Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) ELISA Kit employs a two-site sandwich ELISA to quantitate TIMM44 in samples. An antibody specific for TIMM44 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIMM44 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMM44 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMM44 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.