TMEM53 has no paralogs but is predicted to have many orthologs across eukaryotes. The secondary structure of TMEM53 is predicted to consist of alternating pairs of alpha helices and beta sheets.The function of TMEM53 is not fully understood. It contains a domain of unknown function, DUF829, which is approximately 240 amino acids long. This domain has not been found in proteins other than TMEM53 and its orthologs.Based on human and mouse EST profiles and a human tissue GEO profile, TMEM53 appears to be expressed ubiquitously at low levels in both normal and cancerous tissues.Transmembrane protein 53 has no paralogs. It does, however, have orthologs extending throughout eukaryotes, from primates to amoeba.
Mouse Transmembrane protein 53 (TMEM53) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM53 in samples. An antibody specific for TMEM53 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM53 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM53 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM53 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Transmembrane protein 53 (TMEM53) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-transmembrane-protein-53-tmem53-elisa-kit-kte70179/
bio-equip.cn
中文
