The serum level of amylase (sAm) is commonly used as a biochemical marker for diagnosis and management of pancreatic disorders . Amylase is also one of only a few serum enzymes that are detectable in urine due to its small size and glomerular filtration . However, the use of the urine level of amylase (uAm) is limited in practice , because the diagnostic ability of uAm is inferior to that of sAm . In a few reports, uAm has been used as a marker after endoscopic retrograde cholangiopancreatography or pancreas transplantation . The amylase creatinine clearance ratio (ACCR) is an index that uses uAm. ACCR is known to increase during pancreatitis; however, it has little diagnostic value because of its low specificity and sensitivity .
Human Urinary creatinine (UCR) ELISA Kit employs a two-site sandwich ELISA to quantitate UCR in samples. An antibody specific for UCR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUCR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Urinary creatinine (UCR) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-urinary-creatinine-ucr-elisa-kit-kte62727/
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