Dual specificity mitogen-activated protein kinase kinase 5 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5.This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.Expressed in many adult tissues. Abundant in heart and skeletal muscle.
Human Dual specificity mitogen-activated protein kinase kinase 5 (MAP2K5) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP2K5 in samples. An antibody specific for MAP2K5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP2K5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP2K5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP2K5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Dual specificity mitogen-activated protein kinase kinase 5 (MAP2K5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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