Centromere Protein B is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA.This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
Human Centromere Protein(CENP-B) ELISA Kit employs a two-site sandwich ELISA to quantitate CENPB in samples. An antibody specific for CENPB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCENPB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CENPB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CENPB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Centromere Protein(CENP-B) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-centromere-protein-cenp-b-elisa-kit-kte62684/
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