FGL2 is a 439 amino acid secreted protein that is similar to the β- and γ-chains of fibrinogen. The carboxyl-terminus of the encoded protein consists of the fibrinogen-related domains (FRED). The encoded protein forms a tetrameric complex which is stabilized by interchain disulfide bonds.This protein may play a role in physiologic functions at mucosal sites.Fgl2 is a protein that exhibits pleiotropic effects within the body and is an important immune regulator of both innate and adaptive responses. The protein exists as both a type II transmembrane protein found on the surface of macrophages and endothelial cells and can be constitutively secreted by both CD4+ and CD8+ T cells.
Human Fibroleukin (FGL2) ELISA Kit employs a two-site sandwich ELISA to quantitate FGL2 in samples. An antibody specific for FGL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Fibroleukin (FGL2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-fibroleukin-fgl2-elisa-kit-kte62664/
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