Fibronectin (Fn) is a large modular glycoprotein that is found as a polymeric fibrillar network in the extracellular matirx (ECM) and as soluble disulfide-linked dimeric protomers in plasma and other body fluids.The protein subunit is made up of three types of homologous structural repeats termed Fn type I, type II, and type III repeats. Multiple isoforms of the protein formed by alternative splicing at numerous sites, resulting in insertions of extra type III domains (EDA and ECB) or parts of the variable type III connecting segment (V/IIICS), have been identified. Fibronectin is a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, and many integrin receptors. It is involved in multiple cellular processes including cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling.
Human Cellular fibronectin (cFn) ELISA Kit employs a two-site sandwich ELISA to quantitate cFn in samples. An antibody specific for cFn has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anycFn present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cFn is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cFn bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Cellular fibronectin (cFn) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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