The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B. BLVRB is found predominantly in fetal liver. BLVRA is a major component of human adult liver and is identical to the enzyme previously reported as biliverdin reductase.The FLR cDNA, which appeared to be identical to the BLVRB cDNA, encodes a deduced 206-amino acid protein. Western blot analysis of rat tissues showed highest levels of FLR in the erythrocytes and liver. Northern blot analysis detected a 1.1-kb transcript in human tissues. that flavins are the effective electron acceptor of the enzyme and purified FLR to homogeneity.
Human Flavin reductase (BLVRB) ELISA Kit employs a two-site sandwich ELISA to quantitate BLVRB in samples. An antibody specific for BLVRB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBLVRB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BLVRB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BLVRB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Flavin reductase (BLVRB) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-flavin-reductase-blvrb-elisa-kit-kte62434/
bio-equip.cn
中文
