Calnexin is a 91kDa integral protein of the endoplasmic reticulum. It consists of a large N-terminal calcium-binding lumenal domain, a single transmembrane helix and a short, acidic cytoplasmic tail. Calnexin is one of the chaperone molecules, which are c烀̘灰̘
Human Calnexin (CANX) ELISA Kit employs a two-site sandwich ELISA to quantitate CANX in samples. An antibody specific for CANX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCANX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CANX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CANX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Calnexin (CANX) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-calnexin-canx-elisa-kit-kte62431/
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