DPP10 encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Mutations in this gene have been associated with asthma. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. DPP10 lacks the active-site serine, which is substituted with a glycine residue. Database analysis suggested the presence of a second DPP10 transcript. DPP10 shares 48% and 51% amino acid identity with the short and long DPP6 isoforms, respectively.
Human Inactive dipeptidyl peptidase 10 (DPP10) ELISA Kit employs a two-site sandwich ELISA to quantitate DPP10 in samples. An antibody specific for DPP10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPP10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPP10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPP10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Inactive dipeptidyl peptidase 10 (DPP10) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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