Human G antigen 7 (GAGE7) ELISA Kit,Abbkine_specification/price/image

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Many human tumors express antigens that are recognized in vitro by cytolytic T lymphocytes (CTLs) derived from the tumor-bearing patient. The MAGE (melanoma-specific antigen) and GAGE (G antigen) gene family members encode such antigens. To screen for genes associated with cancer progression, Chen et al. (1998) performed differential display PCR using sublines of the nontumorigenic, prostate-specific antigen (PSA)-secreting cell line LNCaP that differed in androgen sensitivity and metastatic potential. They isolated 2 members of the GAGE family, GAGEB1 and GAGE7. GAGE7 encodes a 117-amino acid polypeptide that contains 3 potential casein kinase phosphorylation sites, 2 potential protein kinase C phosphorylation sites, and 1 potential N-myristoylation site. Levels of GAGE7 mRNA were not correlated to the degree of tumorigenicity of prostate cancer cell lines.

Human G antigen 7 (GAGE7) ELISA Kit employs a two-site sandwich ELISA to quantitate GAGE7 in samples. An antibody specific for GAGE7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGAGE7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAGE7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAGE7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human G antigen 7 (GAGE7) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.