Human Protein-S-isoprenylcysteine O-methyltransferase (ICMT) ELISA Kit,Abbkine_specification/price/image

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Protein-S-isoprenylcysteine O-methyltransferase is the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined.In vitro transfection assays showed that ICMT is the functional human homolog of Ste14. Northern blot analysis detected ubiquitous expression of a 3.6-kb transcript. Transfection studies in Chinese hamster ovary (CHO) and COS-1 cells localized ICMT to the endoplasmic reticulum and nuclear membrane, but not to the plasma membrane.

Human Protein-S-isoprenylcysteine O-methyltransferase (ICMT) ELISA Kit employs a two-site sandwich ELISA to quantitate ICMT in samples. An antibody specific for ICMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyICMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ICMT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ICMT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Protein-S-isoprenylcysteine O-methyltransferase (ICMT) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-protein-s-isoprenylcysteine-o-methyltransferase-icmt-elisa-kit-kte62383/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.