MARLIN1 interacts strongly with GABBR1 and that the 2 proteins colocalize in the same subpopulation of neurons in the cortex and hippocampus of the rat brain. Further study showed that MARLIN1 was more prominent in the central region of the cell body and in proximal segments of neuronal projections. It formed discrete granules in hippocampal neurons grown in cell culture. The deduced 626-amino acid human protein contains 3 coiled-coil regions, 2 of which include predicted leucine zipper motifs. The protein shares 95 to 98% sequence identity with the rat and mouse orthologs. Northern blot analysis of rat tissues detected abundant expression in brain and moderate expression in testis. Immunoblot analysis demonstrated an approximately 80-kD human protein.
Human Janus kinase and microtubule-interacting protein 1 (JAKMIP1) ELISA Kit employs a two-site sandwich ELISA to quantitate JAKMIP1 in samples. An antibody specific for JAKMIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyJAKMIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for JAKMIP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of JAKMIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Janus kinase and microtubule-interacting protein 1 (JAKMIP1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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