Human Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (MGAT1) ELISA Kit,Abbkine_specification/price/image

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There are over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The exon includes most of the 5-prime untranslated region, the complete coding sequence, and the complete 3-prime untranslated region. Southern blot analysis indicated that the gene exists in single copy in the human genome, and study of human-hamster somatic cell hybrids indicated that the gene is located on chromosome 5. GlcNAc-T I is expressed as 2 mRNAs of 3.1 and 2.7 to 3.0 kb in all tissues tested, although only the 3.1-kb mRNA is seen in brain, and expression levels are low.

Human Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (MGAT1) ELISA Kit employs a two-site sandwich ELISA to quantitate MGAT1 in samples. An antibody specific for MGAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMGAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MGAT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MGAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (MGAT1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.