TRIF is primarily active in the spleen and is often regulated when MyD88 is deficient in the liver, indicating organ-specific regulation of signaling pathways. Curiously, there is a lack of redundancy within the TLR4 signaling pathway that leads to microbial evasion of immune response in the host after mutations occur within intermediates of the pathway. Three TRAF-binding motifs present in the amino terminal region of TRIF are necessary for association with TRAF6. Destruction of these motifs reduced the activation of NF-κβ, a transcription factor that is also activated by the carboxy-terminal domain of TRIF in the upregulation of cytokines and co-stimulatory immune molecules. This domain recruits receptor-interacting protein (RIP1) and RIP3 through the RIP homotypic interaction motif.
Human T-lymphoma invasion and metastasis-inducing protein 1 (TIAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate TIAM1 in samples. An antibody specific for TIAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human T-lymphoma invasion and metastasis-inducing protein 1 (TIAM1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-t-lymphoma-invasion-and-metastasis-inducing-protein-1-tiam1-elisa-kit-kte62328/
bio-equip.cn
中文
