EPH receptor A3 (ephrin type-A receptor 3) is a protein belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats.The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.
Human Ephrin type-A receptor 3 (EPHA3) ELISA Kit employs a two-site sandwich ELISA to quantitate EPHA3 in samples. An antibody specific for EPHA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEPHA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPHA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPHA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Ephrin type-A receptor 3 (EPHA3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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