Alpha-N-acetylgalactosaminidase (EC 3.2.1.49) is a lysosomal glycohydrolase that cleaves alpha-N-acetylgalactosaminyl moieties from glycoconjugates.The cDNA encodes a 411-amino acid protein with a 17-residue signal peptide and 6 putative N-glycosylation sites. Northern blot analysis detected 2 mRNA transcripts of 3.6 and 2.2 kb. Sequence analysis revealed striking similarities between the NAGA gene and exons 1-6 of the alpha-galactosidase A gene (GLA), suggesting that the 2 genes evolved by duplication and divergence from a common ancestral locus. Wang and Desnick (1991) also pointed to remarkable amino acid identity between the NAGA and GLA genes.
Human Alpha-N-acetylglucosaminidase (α-NAG) ELISA Kit employs a two-site sandwich ELISA to quantitate NAGLU in samples. An antibody specific for NAGLU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAGLU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAGLU is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAGLU bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Alpha-N-acetylglucosaminidase (α-NAG) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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