Entactin (or nidogen) is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans ( heparan sulfate and glycosaminoglycans), laminin and fibronectin. Entactin (or nidogen) is a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix.Entactin, a 150-kD sulfated glycoprotein, is a major component of basement membranes and forms a highly stable noncovalent complex with laminin. Durkin et al. (1988) derived the complete amino acid sequence of mouse entactin from sequencing of cDNA clones.
Human Nidogen (NID) ELISA Kit employs a two-site sandwich ELISA to quantitate NID1 in samples. An antibody specific for NID1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNID1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NID1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NID1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Nidogen (NID) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-nidogen-nid-elisa-kit-kte62218/
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