Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. The protein encoded by DEFB114 is a beta-defensin with antimicrobial activity against E. coli, S. aureus, and C. albicans. The encoded protein also binds and neutralizes lipopolysaccharide (LPS), a factor involved in inflammatory diseases and male reproductive issues.
Human Beta-defensin 114 (DEFB114) ELISA Kit employs a two-site sandwich ELISA to quantitate DEFB114 in samples. An antibody specific for DEFB114 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDEFB114 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFB114 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFB114 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Beta-defensin 114 (DEFB114) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-beta-defensin-114-defb114-elisa-kit-kte62118/
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