A null ydeQ mutation slightly suppresses the motility defect of a yhjH null mutant at 28C and 37C; a yedQ mutation is needed in addition to yegE (28C) or yegE yeaJ (37) to restore full motility to the yhjH mutant (Pesavento, 2008).YedQ has a C-terminal GGDEF domain. RpoS regulon (Weber, 2006; Pesavento, 2008).
Human Probable diguanylate cyclase YedQ (DGC) ELISA Kit employs a two-site sandwich ELISA to quantitate DGC in samples. An antibody specific for DGC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDGC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DGC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DGC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Probable diguanylate cyclase YedQ (DGC) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-probable-diguanylate-cyclase-yedq-dgc-elisa-kit-kte62088/
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