DGKI is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by DGKI is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration.
Human Diacylglycerol kinase iota (DGKI) ELISA Kit employs a two-site sandwich ELISA to quantitate DGKI in samples. An antibody specific for DGKI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDGKI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DGKI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DGKI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Diacylglycerol kinase iota (DGKI) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-diacylglycerol-kinase-iota-dgki-elisa-kit-kte62079/
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