Human Disks large-associated protein 3 (DLGAP3) ELISA Kit,Abbkine_specification/price/image

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Using a segment of rat Fak (PTK2) to screen an adult human brain cDNA library, Bongiorno-Borbone et al. (2005) cloned DLGAP3, which they called SAPAP3. The deduced protein contains 977 amino acids. By Northern blot analysis, Takeuchi et al. (1997) found brain-specific expression of rat Sapap3. The rat protein localized to dendrites and cell bodies and was enriched in synaptosomal membrane fractions.By mutation analysis, Bongiorno-Borbone et al. (2005) demonstrated that the N-terminal half of human SAPAP3 interacted with a C-terminal segment of rat Fak. They also found that SAPAP3 interacted with Pyk2 (PTK2B) in in vitro pull-down assays. In rat forebrain lysates, part of Fak and Pyk2 had a similar subcellular distribution as Sapap3 and Sap90/Psd95, which supported their functional association.

Human Disks large-associated protein 3 (DLGAP3) ELISA Kit employs a two-site sandwich ELISA to quantitate DLGAP3 in samples. An antibody specific for DLGAP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLGAP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLGAP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLGAP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Disks large-associated protein 3 (DLGAP3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.