DLGAP4 is a membrane-associated guanylate kinase found at the postsynaptic density in neuronal cells. It is a signaling molecule that can interact with potassium channels and receptors, as well as other signaling molecules. The protein encoded by this gene can interact with PSD-95 through its guanylate kinase domain and may be involved in clustering PSD-95 in the postsynaptic density region. The encoded protein is one of at least four similar proteins that have been found. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. SAPAPs are specifically expressed in neuronal cells and enriched in the PSD fraction. SAPAPs induce the enrichment of PSD-95/SAP90 to the plasma membrane in transfected cells.
Human Disks large-associated protein 4 (DLGAP4) ELISA Kit employs a two-site sandwich ELISA to quantitate DLGAP4 in samples. An antibody specific for DLGAP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLGAP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLGAP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLGAP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Disks large-associated protein 4 (DLGAP4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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