Raymond et al. (1999) sought other genes with DM domains within the minimal sex reversal region of 9p. Using a degenerate PCR strategy, they isolated a cDNA clone encoding a protein with a DM domain similar to that of DMRT1 from a human adult testis cDNA library. This cDNA, DMRT2, encodes a 226-amino acid peptide sharing 80% amino acid identity with DMRT1 in the core region of the DM domain.The 2 proteins also have an additional 29-amino acid conserved DM domain region.The authors concluded that the results provide further support for the presence of a sex-determining gene(s) on distal 9p and favor the possibility of DMRT1 and/or DMRT2 being the sex-determining gene(s).
Human Doublesex- and mab-3-related transcription factor 2 (DMRT2) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRT2 in samples. An antibody specific for DMRT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Doublesex- and mab-3-related transcription factor 2 (DMRT2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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