DSE is a tumor-rejection antigen.Two transcript variants encoding the same protein have been found for this gene.Sequence analysis predicted that the 958-amino acid SART2 protein contains a signal peptide, 5 potential N-glycosylation sites, and 2 C-terminal hydrophobic potential transmembrane regions. Northern blot analysis revealed expression of a 4.0-kb transcript in all squamous cell carcinoma (SCC) cell lines and in some adenocarcinoma cell lines tested. Expression was detected in all normal tissues tested, with highest expression in kidney and ovary and lowest expression in colon, thymus, and brain. Immunofluorescence microscopy demonstrated intracellular expression of SART2 in Golgi, endoplasmic reticulum, and secretory vesicles, but not on the plasma membrane.
Human Dermatan-sulfate epimerase (DSE) ELISA Kit employs a two-site sandwich ELISA to quantitate DSE in samples. An antibody specific for DSE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDSE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DSE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DSE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Dermatan-sulfate epimerase (DSE) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-dermatan-sulfate-epimerase-dse-elisa-kit-kte61976/
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