Human Endothelin-converting enzyme 2 (ECE2) ELISA Kit,Abbkine_specification/price/image

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Endothelin-converting enzymes, such as ECE2 are type II metalloproteases that generate functionally pleiotropic members of the endothelin vasoactive peptide family.The deduced protein contains 765 amino acids. ECE2 had an apparent molecular mass of 81 kD by SDS-PAGE.The ECE2A variant contains exons 1A and 2A and encodes a deduced 787-amino acid protein with a calculated molecular mass of 89.1 kD. ECE2A has an intracytoplasmic tail, a transmembrane domain, and a large extracellular catalytic domain with a zinc-binding motif conserved in Zn(2+) metalloproteases. It has 9 N-glycosylation sites. The ECE2B variant contains exons 1B and 2B and encodes a deduced 682-amino acid protein with a calculated molecular mass of 86.4 kD. ECE2B differs from ECE2A only in the intracytoplasmic tail.

Human Endothelin-converting enzyme 2 (ECE2) ELISA Kit employs a two-site sandwich ELISA to quantitate ECE2 in samples. An antibody specific for ECE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyECE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Endothelin-converting enzyme 2 (ECE2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.