Human Delta (3,5)-Delta (2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1) ELISA Kit,Abbkine_specification/price/image

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Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial is a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, Δ3,5-Δ2,4-dienoyl-CoA isomerase, contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Human Delta (3,5)-Delta (2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1) ELISA Kit employs a two-site sandwich ELISA to quantitate ECH1 in samples. An antibody specific for ECH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyECH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Delta (3,5)-Delta (2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-delta-35-delta-24-dienoyl-coa-isomerase-mitochondrial-ech1-elisa-kit-kte61957/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.