Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1) ELISA Kit,Abbkine_specification/price/image

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EGF-containing fibulin-like extracellular matrix protein 1 spans approximately 18 kb of genomic DNA and consists of 12 exons. Alternative splice patterns in the 5' UTR result in three transcript variants encoding the same extracellular matrix protein. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. The cDNA for this gene, designated 'fibrillin-like' (FBNL), was isolated from a fibroblast cDNA library. The FBNL cDNA probe detected 2 transcripts of 2.2 and 3.0 kb in mRNA from multiple tissues. The FBNL gene is expressed in many tissues but it is not expressed in brain and lymphocytes. the amino acid sequence of the FBNL gene is 36.3% identical to FBN1 and 35.4% identical to FBN2 and that FBNL contains repeated cbEGF-like domains, a cardinal motif of FBN genes.

Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1) ELISA Kit employs a two-site sandwich ELISA to quantitate EFEMP1 in samples. An antibody specific for EFEMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEFEMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFEMP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFEMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.